IEF works by applying an electric field to protein within a pH gradient. The proteins separate as they migrate through the pH gradient in response to the applied voltage. When a protein reaches a pH value that matches its pI, its net electrical charge becomes neutral, and stops migrating. In this way, each protein in a sample becomes "focused" according to its pI.
Anaerobe- A microorganism that can grow in the absence of O2 Androgenesis- Development of Isoelectric focussing from male gametophytes Aneuploidy - An abnormal condition of chromosomes, differing from the usual diploid constitution. This may be due to a loss or gain of chromosomes.
Annealing - The pairing of complimentary single strands of DNA to form a double helix.
Apoptosis - Programmed cell death Assisted reproductive technology ART - The manipulations of reproduction in animals and humans.
Auxins - A group of plant growth regulators which are involved in cell elongation, root initiation etc. Bacteriophage - A virus that infects a bacterium, also called as phage. Base pair bp - The hydrogen bonded structure formed Isoelectric focussing two complementary nucleotides i.
Bioaccumulation - Concentration of a chemical agent e. DDT in the increasing amount in the organisms of a food chain. Bioaugmentation - The addition of microorganisms to waste sites so that the hazardous wastes are rendered harmless.
Biochemical oxygen demand BOD - The oxygen required to meet the metabolic needs of aerobic organisms in water containing organic compounds. Biodegradation - Biological transformation of organic compounds by living organisms, particularly the microorganisms.
Biofertilizer - Commercial preparation of microorganisms by using which the nitrogen nd phosphorus level and growth of plants increase. Biofilteration - The process of removing complex wastes from domestic and industrial sources by using microorganisms. Biohazards - The accidents or risks associated with biological materials.
Bioleaching - The use of bacteria to recover, valuable metals from ores. Biolistics - The process of introducing DNA into plants and animal cells, and organelles by bombardment of DNA-coated pellets under pressure at high speed. This is also called as microprojectile bombardment. Biomass - The organic mass that can be used as a source of energy.
Biomass also refers to the cell mass produced by a population of living organisms. Biometry - Application of statistical methods to study biological problems. Biopesticides - The toxic compounds produced by living organisms that can specifically kill a particular pest species.
Bioprocess technology - A more recent usage to replace fermentation technology that involves large scale cultivation of microorganisms for industrial purposes.
Bioreactor - A growth chamber or a vessel for cells or microorganisms. The cells or cell extracts carry out biological reactions in a bioreactor.
Bioremediation - The process of using living organisms to remove contaminants, pollutants, or unwanted substances from soil or water.
Biosensor - An electronic device that uses biological molecules or cells to detect specific compounds. Biosorption - The process of microbial cell surface adsorption of metals.
Biostimulation - Addition of specific nutrients to enhance the growth of naturally occurring microorganisms that convert toxic compounds to non-toxic compounds.
Biotechnology - The applications of biological principles, organisms and products to practical purposes. Biotic stress - The stress caused to plants by insects, pathogens viruses, fungi, bacteriawounds etc.
Biotransformation - The use of biological systems for the conversion of biomolecules. Biodiversity - The variability among the living organism from all sources, soil, water, air, extreme habitat or associated with organisms.Isoelectric focusing is an electrophoretic method in which proteins are separated on the basis of their pIs ().
It makes use of the property of proteins that their net. Capillary isoelectric focussing cIEF Coriolis Pharma is specialized in providing stability indicating protein analytical methods, including capillary isoelectric focusing for monitoring charge heterogeneity e.g.
protein deamidation. The first dimension in a 2-D gel electrophoresis experiment involves the separation of proteins according to their isoelectric point (pI) by isoelectric focusing (IEF). IEF works by applying an electric field to protein within a pH gradient.
The proteins separate as they migrate through the . Isoelectric Focusing. Mathy and Sluse, ). Since the net charge of the proteins depends on pH, electrophoretic is attained by pH changes in this method.
The net charge of the proteins is the sum of the negative and positive charges at the amino acid chain regions. Isoelectric focusing definition is - an electrophoretic technique for separating proteins by causing them to migrate under the influence of an electric field through a medium (such as a gel) having a pH gradient to locations with pH values corresponding to their isoelectric points.
Experience maximum resolution capillary isoelectric focusing and fast capillary zone electrophoresis that only SCIEX Separations can provide.